Viral transduction of cells of the nervous system

Neuroscientist Dr.Doping tells about protein knockout, transgenic animals and viral delivery system.For some studies, scientists used the protein knockout? What are the challenges faced by professionals working with transgenic animals? And how the viral genetic material delivery system?

The development of modern science has reached phase of living organisms. People can make transgenic animals, which then can be used for practical purposes .They are also widely used for scientific research. To investigate the function of any protein, it is necessary to make a "knockout" actually remove the gene encoding this protein. Thus, it turns out the animal deprived of functions. People often use a knockout at the level of the whole organism. There are various possibilities: gene can be removed at some specific part, as may be the whole body and thus to investigate its function.

There are several types of viral deliveries systems: based on retrovirus, adeno-associated virus based, lentiviruses. The general sense is that there are viral particles carrying the desired genetic material. As a rule, this particle is not able to replicate in the body, a weapon only once shooting. It is necessary to carry out preliminary work to collect the virus particles in other cell lines, and only then it is possible to work with them. You can then move to the level of the whole organism and to work with experimental animals. Is now undergoing clinical studies using viral particles as vehicles for gene therapy.

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In order to obtain functional viral particles, it is necessary to take a line packers cells which are artificially introduced virus components. You can make 4 pieces For lentiviral particles that then, like a mosaic, gather the finished virus particle. It can be used in experiments.